removal of IgG from fc receptors
Antony Bakke (bakkea@ccmail.ohsu.edu)
Mon, 10 Apr 95 09:36:47 PST
Removal of IgG from Fc receptors can be accomplished in one of
two ways: 1) use low pH (3-4) as another respondant replied, or
2) use warm washes. This is less toxic to the cells. Incubate
cells for 30 min at 37 degrees C in media with 10% fetal calf
serum. Then wash 2-3 times by centrifugation with the same
warmed media.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
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