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Using PI staining of mesenchymal-derived cells (osteoblasts), I have
noticed some variations in cell cycle distribution with and without a
stimulus. I would like to further separate the G1/G0 cells into a
G0, early G1 and late G1 sub population. Likewise, I would also like
to separate the G2/M population into G2 and M subpopulations. I have
tried discriminating on the basis of side-scatter vs PI signal after
heat denaturation and chromosomal unwinding but can not separate the
populations any further.
Has anyone tried this or have any familiarity with separation of
cells into more defined subpopulations? I can not use a time course
assay as the response is only noted at a particular point in time.
This must also be performed on an asynchonous population of cells in
log-phase growth. If there are any references that are of use, I
would be highly appreciative.
Thank you in advance for all your input. Replies that are useful
will be posted at a later date.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web