Dear Birger Christensson,
We have done it on a FACSTAR+.
We used 20 ul Hoechst 33342 (MEMBRANE PARMEABLE DYE) for 2 ml cell
suspension. ncubate for 1 our, 37 Celsius in the Dark. You have to exitate
with uv.
I understand that you have a FACSCAN so you don't have uv.
There is a new dye called YOYO 1 from Becton Dickenson. For more information
about this you should contact:
Maarten van de Keur
Academic Hospital Leiden CKHL
tel: +31 71 261783
or: +31 71 276831
Sincirely, Ron.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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