From: Robert C. Leif
Vice President Newport Instruments
>DOES ANYONE KNOW OF A SUPPLIER FOR EUROPIUM CHELATES SUITABLE FOR
>CONJUGATING TO ANTIBODIES. WE ARE INTERESTED IN USING THESE REAGENTS FOR
>TIME RESOLVED FLUORESCENCE IN FLOW FOR DETECTING VERY SMALL PARTICLES.
>
>HAS ANYONE GOT EXPERIENCE WITH TIME RESOLVED FLUORESCENCE IN FLOW.
>
>ANY SUGESTIONS FOR FLUOROCHROMES WITH LONG FLUORESCENCE LIFETIMES.
>REGARDS,
>
>GRAHAM VESEY
>SCHOOL OF BIOLOGICAL SCIENCE
>MACQUARIE UNIVERSITY
>SYDNEY, NSW 2050
>AUSTRALIA.
>TEL-612 850 8150 FAX-612 850 8174
>
The Vallarino Leif Macrocycle is available from:
Attn: Dan Flowers
Director of R & D
Rob Sternfeld, Director of Marketing
Research Organics Inc
The Producers
4353 East 49th Street
Cleveland, Ohio 44125
(216) 883-8025, (800) 321-0570; FAX 216 883-1576 Telex 98-5247
74222.1506@compuseve.com
I recommend that you run your instrument with an ethanol or methanol sheath.
Since the lifetime is about 500 microseconds, the flow rate should be very slow.
Research Organics will supply the macrocycles and I will provide the
technical support.
>
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web