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I have several questions.
Could you reply some of them, if possible ?
1. Can ELF from Molecular Probes be used in flow cytometry ?
I want to stain intracellular proteins with AP-conjugated antibodies.
Amplification of the signals using ELF is available ?
Our EPICS ELITE ESP is equipped with He-Cd 325nm line. I think ELF
would be excited by 325nm UV.
Or, must I try to use Fast Red TR or something other substrates ?
2. Are TOTO-3 and TO-PRO-3 excited by Ar 488nm line ? I mean, when
analysing cell surface antigens by FITC, PE and PerCP-labeled
antibodies, can TOTO-3 and TO-PRO-3 be used as live/dead cell
discriminators like PI using He-Ne 633 nm simultaneously ?
3. Is there anybody who uses AMCA ?
Is this dye good for three or four color analysis ?
Any comments and replies would be appreciated.
Thank you.
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Fumio Ike, PhD. E-mail:fike@rkna50.riken.go.jp
Animal and Cellular Systems Laboratory TEL:+81-48-462-1111(ext5613)
The Institute of Physycal and Chemical Research (RIKEN) FAX:+81-48-462-4681
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web