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Hello,
I am looking for references and leads on sorting the hybrid product from a
cell fusion...I recall some work that was done in isolating hybridomas from
PEG fusions, where each fusion partner was labeled with a different
fluorochrome (e.g., the immortalized line in green, the Ag presenting cell
in red) and the resulting dual color hybrid sorted. One of our local folks
remembers a Dutch(?) group that used different colored beads, but I have
been unable to locate a reference....can you help me?
Thanks in advance.
Bill Hyun
Director, Lab for Cell Analysis
University of California, San Francisco
Tel: (415)476-2631
FAX: (415)476-8218
email: hyun@dmc.ucsf.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web