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Date: 2/11/94 2:04 PM
To: Donna Gandour
From: Dan Schaeffer
- From flowcyt.cyto.purdue.edu!kelley Fri Feb 11 13:13:53 1994
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To: cytometry@flowcyt.cyto.purdue.edu
From: SRESS@uctgsh1.uct.ac.za
Date: 11 Feb 94 16:15:31 SAST-2
Subject: Re: cytoplasmic staining
X-Pmrqc: 1
Priority: normal
X-Mailer: Pegasus Mail v2.3 (R4).
BallasZ@intmed-po.int-med.uiowa.edu wrote:
I have been trying to develop a reproducible and specific method for
simultaneous surface and cytoplasmic staining. I have had problems with
most of the published methodology especially when attempting to use
an isotype control.
>>>I would also like to hear about successful methods for this-please
post to the discussion group as well! In fact, I would be grateful if
anyone can share a recipe for successful detection of intracellular
IL-4 and IFN-gamma: we have not been able to detect these (or IL-2)
using a variety of protocols on human cells in bulk culture, or in
human clones. Activation has been tried with PHA, PPD, PMA and solid
phase OKT3. Any offers?
Stan.
Stanley Ress
Clinical Immunology laboratory
H47 Old Main Bldg
Groote Schuur Hospital and UCT
Cape Town, 7925
South Africa
TEL:INTERN. + 021-7946176
FAX: " + 021-4486815
e-mail: SRESS@uctgsh1.uct.ac.za
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web