Figure 6: Typical histogram of Escherichia coli treated with 70% ETOH and stained with the BacLight Kit. Region 4 contains non-viable bacteria and is used as a dead control.

Figure 7: Two color histogram of Escherichia coli incubated at room temperature and stained with BacLight Kit. Cells in region 2 are viable and used as a live control.

Figure 8: Bivariate contour plot of Escherichia coli heat incubated at 60 degrees Celsius and stained with BacLight Kit. Cells in region 3 are non-viable.

Figure 9: Typical pattern of Escherichia coli treated with Gentamicin (0.5 ug/ml) and stained with BacLight Kit. Cells in region 2 are viable. Regions 1,3,4, and 5 are non-viable.

Figure 10: Contour plot of Escherichia coli treated with Gentamicin (16 ug/ml) and stained with the BacLight Kit. Region 2 contains viable cells. Regions 1, 3, 4, and 5 contain non-viable cells. Percentages increased in all non-viable regions as the dosage of Gentamicin was increased and the incubation duration was held constant. As the percentages of non-viable bacteria increased the percent of viable cells in region 2 decreased.

Back to Flow Cytometry and Microbiology Introductory Page

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu