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That aside, it may be possible to block the DNA sites (I see GC-like specificity
on chromosomes) and use TO like pyronin. In answer to the specific questions,
the dye is nearly non-fluorescent when not bound to nucleic acids, excites well
at 488 and emits in FL1 with more FL2 that is manageable but a little more than
you would get for fluorescein (see Cytometry 20:218 for some data on SY-38, a TO
derivative). The very high fluorescence at saturation (DNA and RNA) will swamp
FL2 and probably FL3. Spectra should resemble those of TO-PRO-1 (another TO
derivative) in the Molecular Probes catalog (comments Martin?).
Subject: fluorescent RNA specific dyes
Date: 5/23/96 10:33 AM
Does anyone have or know about recent data on a comparison
of the characteristics of different RNA specific dyes
excitable at 488 or 633/635 nm?
What is the specificity (RNA dependent increase or spectral shift of
fluorescence) of thiazole orange in comparison to auramine O,
pyronine Y or others?
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