Section 12
Confocal Assistant,
Dr. Todd Brelje 
DIP, Dr. Angel Martinez Nistal, Univ. Oviedo
please read this.
Human Chondrocytes in culture - "The Greedy Chondrocyte!" WARNING: This file is 8 megabytes. This time lapse video depicts the phagocytic abilities of chondrocytes. These cells are 2nd passage human chondrocytes grown in monolayer. One image was collected every 30 seconds for 1 hour. Note the pseudopod as it pulls away from a piece of debris located in the media. The images were collected on the Bio-Rad MRC 1024 confocal microscope using the transmission detector and inverted Nikon 300 scope and a Brook Industries heated stage. The file sectcho3.pic can only be viewed using Confocal Assistant (see above)
Cultured Rat Endothelial Cells - "The Busy Mitochondria!" [WARNING: This file is 1 megabyte.] Endothelial cells in culture, loaded with Rhodamine 123 to label mitochondria. The is a time lapse video of images every 10 seconds. The file TI1.pic can only be viewed using Confocal Assistant (see above)
Calcium Flux - Cultured Rat Endothelial Cells WARNING: This file is 1 megabyte. The file wade.pic can only be viewed using Confocal Assistant (see above). Cells are cultured in chambers and run on the inverted confocal microscope. Cells are loaded with Indo-1 (3uM) for 20 min, followed by stimulation with Ionomycin (3 uM). Ratio images are collected over time.Credits: Dr. J. Paul Robinson, Purdue University Cytometry Laboratories
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