BrdU-Proliferation assay question

Andreas Schroeder (h82@ix.urz.uni-heidelberg.de)
Thu, 26 Sep 1996 17:51:24 +0200

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Is anybody of you aware of a good (means working) protocol for
a Br-desoxyuridine-FACS-procedure? I have used the B-D-protocol
provided in their monoclonal antibody resource book, but without
success :-(
In particular I am looking for information about the FACS-setup
for aquisition and analysis of cell proliferation and hints
about possible pitfalls in the procedure.

Any help is greatly appreciated.

--
Andreas Schr=F6der

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu