Optical filters
kelley@wh.bayer.com
Tue, 6 Feb 96 09:04:54 -0500
Geoff O. asks about optical filter properties and transmission
spectra. There is a technical tutorial in Cytometry, 9:277-280,
1988 titled "Practical Consideration for the Selection and Use
of Optical Filters in Flow Cytometry". This tutorial addresses
many of the concerns of optical filters and offers some suggestions
as to how to determine the performance of your individual filters.
Filters do have finite lifetimes and they do change their spectra
slowly as they age. Also, sidebands are just a fact of life when
you are dealing with multi-cavity filters so you need to be aware
of those wavelengths when designing multiple dye or multiple laser
protocols.
Keith
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
