Re: DNA/RNA quantification

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I am looking for a protocol for=20
>the reliable quantification of bulk DNA/RNA from tissue. I've tried a=20
>standard digestion and extraction followed by spectrophotometric=20
>readings at 260nm. I feel that I am loosing a lot during the=20
>extraction. Does anyone have any infomation that may help?!?!

Try looking up Nucleic Acids Research 19(15) 4293 for a simple method of=20
extracting DNA from mammalian tissue published 1991.=20

Mikael

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu