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It is my experience that micro airbubbles in the sheet-fluid can cause
problems during sorting, mainly by drifting of the dropformation, thereby
reduse the purity and yield during a run. I therefore, with good success,
routinously de-gass my sheet-fluid to "no boiling" with a vacumpump and a
cold-trap between the pump and sheetfluid (not to destroy the oil-based
vacumpump). This is actually the speed-vac system adapted to fit the fluid
tank. It might be acceptable to use a standard water-jet pump to create the
vacum for de-gassing.
But, of course, if there are leakages in the system (O-rings, tubes, etc.),
this will cause troubles of unstability. These problems should be easy to
evaluate.
Have a merry X-mas and a happy new flowyear !
Kjartan Egeberg
Inst. of Canser Research and Mol. Biol.
University in Trondheim
N-7005 Trondheim
Norway
T.phone: (47) 73 59 86 70
T.fax: (47) 73 59 88 01
E.mail: Kjartan.Egeberg@ifk.unit.no
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