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At 18:15 28/11/95 +0000, Phillip E. Klebba wrote:
>Greetings fellow flow afficionados,
>
>I need help. I have the need and the $$$$$$ to purchase a sorting
>instrument. The machines under consideration are a BD FACScalibur and a
>Coulter Elite ESP. However, the new sorting mechanism of the BD instrument
>makes it difficult to compare it with the Coulter.
>
>Our sorting needs are as follows. We are working with bacteria, and sorting
>cells recognized by MAbs against surface epitopes. In some cases the
>particles of interest may be rare events (10-5). The sorted cells must be
>recovered and re-cultured. I have heard from the BD salesman that the
>Coulter instrument is unreliable for sorting and continually down for
>service, and from the Coulter salesman I have heard remarks about the slow
>sorting speed of the FACScalibur, and low viability of cells recovered with
>the cell concentrator.
>
>Can anyone provide comments and experience that might resolve some of these
>considerations. Although price is a question, the efficacy of the
>instruments is primary. Thanks in advance for any help that you might provide.
>
>PEK
>
>Phillip E. Klebba, Ph. D.
>Associate Professor of Chemistry and Biochemistry
>University of Oklahoma
>Norman, OK 73019
>
>Tel: 405-325-4969
>Fax: 405-325-6111
>Internet: pklebba@aardvark.ucs.uoknor.edu
>
>
>
Peter Openshaw (p.openshaw@ic.ac.uk)
Wellcome Trust Senior Research Fellow in Clinical Sciences,
Respiratory Medicine, Imperial College,
St Mary's Hospital Medical School
London W2 1PG UK. EMail: Direct line: +44 171 594 3853/4
Swithcboard: (0171) 723 1252 ext 3854 Fax: 724 7349
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