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We usually have folks bring loaded cells on ice, then warm to 37
before calcium analysis. This works on most of the cell types we see,
although I can't remember if this ever caused trouble with PBL's.
I think this temperature thing is cell type specific- i remember
seeing Jurkatt cells flux on ice!!
Peter
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Peter A. Lopez (617)632-3179 (voice)
Dana Farber Cancer Institute (617)632-3139 (voice)
Core Flow Cytometry Facility NO FAX!
44 Binney St. Room J-312
Boston,MA 02115 PLOPEZ@sorter.dfci.harvard.edu
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