Platelet activation

Walter Sharp (102675.320@CompuServe.COM)
31 Jul 96 21:37:12 EDT

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Dear All,
I have a query to put to the list for a concensus opinion, if one exists
that is !
I'm sure Vince Shankey will answer this one - but I've lost his address..
A colleague from another hospital is asking me to quantitate IN VIVO activated
platelets in a particular infection.
I have two questions.
1) Can this be simply done by flow cytometry in the opinion of those involved in
"activated platelets".
I know a lot of work has been done on IN VITRO activation but I don't know about
circulatory activation.
2) What is the concensus on the best antibody - CD62P, CD63 or none of the
above.

Thanks
Wal Sharp
SQU
Oman.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu