 |
 |
 |
 |
a. Ease and stability of conjugation
b. Intensity of fluorescence
We use Cy5 conjugated antibodies on a dailey basis excited by a dye
laser tuned to 600nm and emitting 400mW of power. The intensity of
fluorescence is excellent and may possibly be better than APC. We
have never done a direct comparison. Cy5 is the third color of choice
here at the Univ. of Iowa because of its ease of conjugation and good
fluorescence characteristics. We routinely run fluorochrome combinations
of FITC, PE, Cy5, and PI for live/dead discrimination and four color
experiments using FITC, PE, Texas Red, and Cy5. The people who do
Cy5 conjugations here tell me that it is as easy as conjugating FITC
and I have not heard any complaints about stability.
In our hands Cy3 does not work as well as PE. The fluorescence
intensity is much lower.
Hope that helps
Justin Fishbaugh
University of Iowa
Flow Cytometry Facility
internet: justin-fishbaugh@uiowa.edu
|
|
|
|