Intra-cellular antigens (again!)

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Just joined the Mailing List, and would seemed to have missed something on
the labelling of intra-cellular antigens. A Colleague of mine wishes to
look at an antigen localised on the inner surface of mitochondria. Any
ideas on how to get at it? What about preparing mitochondrial preps. first?
What fixation and permeabilisation is best?

Sorry if I am going over the same old ground!

Yours Hopefully,

Don Healey
Snail to:
Dr Don Healey BSc. PhD.
BDA Research Fellow
Dept. of Pathology
Cambridge University
Tennis Court Road
Cambridge CB 1QP
TEL: 0223 333716
FAX: 0223 333914

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu