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> > To: cytometry@flowcyt.cyto.purdue.edu (Cytometry
> Mailing List) > From: Bill_Hyun@dmcmail.ucsf.edu
> > Organization: University of California, SF Division of Molecular Cytometry
> > Date sent: Thu, 15 Sep 1994 15:16:14 PST
> > Subject: heterokaryons
>
> > Subject: Time:1:28 PM
> > OFFICE MEMO heterokaryons Date:9/15/94
> >
> >
> > Hello,
> > I am looking for references and leads on sorting the hybrid product from a
> > cell fusion...I recall some work that was done in isolating hybridomas from
> > PEG fusions, where each fusion partner was labeled with a different
> > fluorochrome (e.g., the immortalized line in green, the Ag presenting cell
> > in red) and the resulting dual color hybrid sorted. One of our local folks
> > remembers a Dutch(?) group that used different colored beads, but I have
> > been unable to locate a reference....can you help me?
> > Thanks in advance.
> >
> > Bill Hyun
> > Director, Lab for Cell Analysis
> > University of California, San Francisco
> > Tel: (415)476-2631
> > FAX: (415)476-8218
> > email: hyun@dmc.ucsf.edu
> >
> More than ten years ago we (Schaap, Verkerk and I) did heterokaryon
> and cybrid sorting using beads and fluorescent dyes.
> Some references are:
> Exptl Cell Res. 120: 444 1979
> Exptl Cell Res. 130: 481 1980
> Exptl Cell Res. 140: 299 1982
> Techniques in Somatic Cell Genetics (Ed. B. Shay) p.81-100. Plenum
> Press.
> I will send you the thesis of Schaap and the Plenum Press photocopy.
>
> Yours,
>
>
> Hans Jongkind
>
>
>
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