Stevan Thomas Lauriault

stevan@lauriault.com; sloreo@hotmail.com; steve@lauritech.com

phone/fax: 416-283-4066

Employment Experience:

FOUNDER, PRESIDENT AND C.E.O

Lauritech Inc.

Biotechnology, Pharmaceutical and Life Sciences Research Service Provider – Flow Cytometry

Scarborough, Ontario

June 22, 2008 – Present

• Developed www.lauritech.com, an SQL-driven PHP-programmed webpage being

hosted out of an on-site APACHE web server.  Developed a username/password secure database-driven web application capable of transferring large data files directly to local, on-site server.

• Database-driven web design, implementation and search engine optimization (SEO).
• Advertisement, client/colleague networking, data transfer via webpage, process development, corporate logo, tri-panel brochure, business card.
• MS Excel and Simply Accounting for financial management, forecasting.
• MS Outlook for contact management, communications.
• NUANS search, articles of incorporation, business name and number registration.  Awarded OSEB grant to assist with funding for preliminary start-up period.
• Certified completion of OSEB program.
• Completed comprehensive business plan and business plan presentation (Powerpoint).
• Full member of International Society for Advancement of Cytometry (ISAC) and Clinical Cytometry Society (CCS). 

CONTRACTOR:  RESEARCH ASSISTANT/TECHNICIAN/TECHNOLOGIST

Sunnybrook Health Sciences Centre

On site: Defence Research and Development Canada - Toronto

Department of National Defence, Canada

April 1, 2006 - April 1, 2008

• Took a leadership role in establishing the laboratory groundwork (first 50 patients, and healthy controls subjects) for the flow cytometry method employed by a multi-hospital clinical trial of hypertonic saline blood substitute, being conducted by the Resuscitation Outcomes Consortium. 
• Developed assays and established instrument settings using single-channel stained controls for DNA, intracellular and membrane-bound biomarkers.
• On-call days and nights to routinely process human whole blood clinical samples from hospital, purify serum/plasma/PAXGene, acquire whole blood on hematology analyzer, and leukocytes on cytometers.
• Routinely prepared solutions, operated pipettes, micropipettes, repeaters, centrifuges, vortexes, water baths, incubators, BD FACSCalibur (w/ sorter) and BD FACSCanto flow cytometers.  BD FACSComp, FACSCanto, CellQuest Pro, FACSDiva software for QC/acquisition. FlowJo 8.7, CellQuest Pro and FACSDiva 5.2 software for analysis.
• Obtained BD FACSCanto training certificate Oct., 2007, Billerica, MA.
• Performed machine calibration, maintenance, trouble-shooting, experiment/template design, test antibody research/ordering, control antibody ordering, isotype/concentration matching, antibody titrations, fluorescence compensation, data acquisition, analysis, patient-specific boolean gating, iterations, population comparisons.   
• Upgraded Software and Hardware. 
• Conducted further analysis of trial results using StatView statistical analysis software.
• Trained users in universal safety precautions, solution preparation, diverse staining techniques (intracellular/surface/DNA), flow cytometry troubleshooting, multitasking, data acquisition and batch analysis.
• Researched advanced cytometry techniques, experimental design (normal cytometry, FACS and ImageStream), purchasing quote generation, group scheduling, hardware/software upgrades, LWA, PC/Mac, carousel setup (automation), MS Windows/OSX, Internet web-browsers, online literature search using EndNotes and full-text search, publication review, MS Outlook, Word, Excel, Powerpoint.
• Took leadership role in designing and implementing human whole blood staining experiments on Amnis ImageStream Cellular Imaging device. Also analyzed and interpreted experimental results.  Coordinated off-site FACS coordination experiments and presented assay development data/results to ROC staff.

RESEARCH TECHNOLOGIST I

The Hospital for Sick Children

Flow Cytometry Core Facility

TORONTO, ON

March 2005 - Sept. 2005 (part-time, temp. contract)

• Daily maintenance, calibration and operation of the BD FACScan and FACSCalibur Flow Cytometers.
• Acquired and analyzed flow cytometry data (1-4 colour) from multiple laboratories using CellQuest Pro and FlowJo software.
• Emulated/Constructed batch analysis template and performed batch analysis using FlowJo software.
• Scheduled and managed assay appointments using FileMaker Pro. Trained users how to acquire and analyze data.

SCIENTIST II (TN Visa title)

Aastrom Biosciences

ANN ARBOR, MI

Sept. - Nov. 2004 (full-time, temp. contract)

• 4-colour analysis of human bone-marrow using Coulter Epics XLMCL flow cytometry with windows-based Expo32 software.
• Multi-panel sample staining and data archiving.

SCIENTIST II (TN Visa title)

Pfizer Inc. - Global Research and Development

ANN ARBOR, MI

April - Sept. 2004 (full-time, temp. contract)

• Optimization of 4-color Lymphocyte sub-setting assay.
• Rodent euthanasia and splenectomy.
• Single-cell suspensions from tissue, cell-staining, flow cytometry using FACSCalibur with CellQuest Pro software, mAb preparations, dilutions, SOPs, databook and notebook recordings, data analysis, validation and PowerPoint presentation.
• Training: Chemical spill safety, General Site Safety, Animal Welfare training.

TEACHING ASSISTANT

University of Windsor, Department of Biology

WINDSOR, ON

Jan. - April 2004 (part-time, semester)

• Assisted in teaching first year biology lab with the department of Biological Sciences while pursuing Hon. B. Sc. in Biochemistry and Biotechnology.

SOFTWARE ENGINEER/VICE PRESIDENT - startup company

InDevPro Inc.

WINDSOR, ON

Feb. - April 2003

• Assisted in family entrepreneurial endeavor.
• learned basics in Database-driven web programming using Dreamweaver MX, Flash MX,

ColdFusion MX, HTML, Java, C++, MySQL server, MS Access.

• Database-driven Webpage design, template production, multitasking and office duties.

CHEMIST/BIOCHEMIST

HydroBiotech Inc.

MIRABEL, QUEBEC

Feb. - May 2002 (full-time, contract)

• Project Management, Excel database input/functions, Chemical Preparation, spectrophotometric analysis, ion-selective electrode maintenance and testing, pH

measurement, flame photometric analysis, blood serum analysis, basic HPLC, bilingual environment (French/English).

RESEARCH ASSOCIATE

Université de Lausanne/ISREC

EPILANGES, SWITZERLAND

July - Sept. 2001 (full-time, stage)

• Assisted with experiments in tumor targeting using an innovative approach.
• In collaboration with ISREC and Ludwig Institute for Cancer Research.
• Bacterial culture, incubation and stimulation.
• Synthesis and purification of inclusion bodies from E.Coli and subsequent Bradford assay

spectrophotometry, SDS-PAGE and BioRad chromatography.

• Performed refolding procedure (dialysis) of soluble MHC class I protein with flu peptide.
• Tumour cell culture, mouse maintenance, (SCID, normal), FACS analysis, ELISPOT and lab duties. For more information, see www.cancerimmunity.org vol. 3 no. 11.

RESEARCH ASSOCIATE

Rigel Pharmaceuticals Inc.

S. SAN FRANCISCO, CALIFORNIA

May - Sept. 2000 (full-time, temp. contract)

• Calculated dilutions for Jurkat T-cell proliferation. Maintained cells in the biohazard cell culture room, organized cloned cells into 96 well plates, BD FACSCalibur w/ CellQuest software operation.  Analyzed data and documented results.

Education:

BACHELOR OF SCIENCE DEGREE, BIOLOGICAL SCIENCE

University of Windsor

Windsor, ON

Sept. 1996 - June, 2000

Hon. B.Sc., BIOCHEMISTRY AND BIOTECHNOLOGY (completed 3^rd year)

University of Windsor

Windsor, ON

Sept. 2004 - May 2005

GRADUATE STUDENT - UNCLASSIFIED

University of Calgary

Calgary, AB

Sept. - Dec., 2000

Developed grant proposal focusing on a bispecific molecule (HLA I/FluA/anti-tumour antigen IgG hybrid) to be used in cancer immunotherapy.  See Lausanne/ISREC in experiences.