3144B Grant Avenue (847) 321-5226
Philadelphia, PA 19114 drjmoore@temple.edu
Objective: To manage a multi-instrument Flow Cytometry Core Facility and facilitate cutting-edge research utilizing polychromatic sorting and analytic techniques. In addition, to promote education among facility users and raise awareness of the potential benefits and novel applications offered by flow cytometric technology.
Experience: TEMPLE UNIVERSITY, Philadelphia, PA
Flow Cytometry Core Facility Manager March 2005 - Present
· Primary operation and maintenance of FACScan, FACSCalibur and FACSAria
· Routinely perform 7-color analysis and sorting on the FACSAria
· Instrument training and education in multicolor flow techniques for facility users
· Software training including CellQuestPro, ModFit and FlowJo
· Advise investigators in experimental design of polychromatic analysis and sorting
· Provide and assist in the development and optimization of flow cytometric protocols
· Contribute to flow cytometry-associated grant preparation
· Perform data analysis and generate publication- and presentation-quality figures
· Prepare educational materials for facility users
· Maintain core facility billing records
BD BIOSCIENCES, San Jose, CA
Research Reagent Sales Specialist Nov 2003 - Feb 2005
· Managed 6 state territory promoting sales of BD flow cytometry products and instrumentation
· FACSArray technical specialist
· Performed product demonstrations and technical presentations
· Provided customers with technical support of flow cytometry products and instrumentation
· Proficiency in ACT6.0 database, and Excel to manipulate and extract sales stats
· Participated in and organized local vendor shows providing both sales and technical support
BD BIOSCIENCES, PHARMINGEN, San Diego, CA
Technical Application Specialist Oct 2001 - Nov 2003
· Pre- and post-sales technical support for BD flow cytometry research reagents
· Developed and presented product and educational seminars on flow cytometry applications
· Provided product demonstrations and training to individual customers and groups at national meetings
· In-house testing and critique of products and instrumentation under development
· Assisted customers in experimental design, and application development and optimization
· Established collaborations with investigators for the licensing and development of new products
· Academic Research Coordinator
3144B Grant Avenue (847) 321-5226
Philadelphia, PA 19114 drjmoore@temple.edu
Research
Experience: NCI, LAB FOR IMMUNE CELL BIOLOGY, Bethesda, MD
· Studied the effects of glucocorticoid production on murine T cell development and peripheral homeostasis
UNIVERSITY OF COLORADO, HSC, Denver, CO
Pre-Doctoral Student, Immunology Aug 1994 - Dec 1999
LOMBARDI CANCER CENTER FLOW CORE, Washington, DC
Application Development Technician Oct 1991 - Aug 1994
· Optimized flow cytometry protocols required by facility users
· Designed methods handbook for facility users
· Prepared flow samples
CELLCO, INC., Kensington, MD
Research Technician Jan 1991 - Oct 1991
· Developed and optimized hollow-fiber bioreactors for the production of conditioned media
· Performed routine tissue culture
Research
Awards: Award for Outstanding Research; 1997 Annual Student Research Forum, University of Colorado, HSC
“Autoimmune T Lymphocytes Exhibit Costimulation-Induced Cell Death that may represent a Novel Pathway.” Moore, J.K., Duke, R.C., and Bellgrau, D.
Education: University of Illinois, C-U, Champaign-Urbana, IL
University of Colorado, Health Sciences Center, Denver, CO
3144B Grant Avenue (847) 321-5226
Philadelphia, PA 19114 drjmoore@temple.edu
Technical
Skills: Proficient in CellQuestPro, DiVA 4.1, ModFit, and FlowJo software, as well as MS Office Programs
Operation of BD instrumentation including: FACScan, FACSCalibur, FACSArray, and FACS Aria
Publications: 1. Voss, Jr., E. W., Moore, J. K., Weidner-McGufficke, K. M., Denzin, L. K., Bedzyk, W. D., and Voss, V. H. Autologous Anti-Metatype Immune Response in Rabbits. Molecular Immunology 29: 241-249, 1992
2. Bellgrau, D., Gold, D., Selawry, H., Moore, J., Franzusoff, A., and Duke, R. C. A Role for CD95 Ligand in Preventing Graft Rejection. Nature 377: 630-632, 1995
3. Moore, J. K., and Bellgrau, D. Promiscuous Activation and Cell Cycle Entry in T Cells from Autoimmune Animals. Transplantation Proceedings 31:1606-1610, 1999
4. Moore, J. K., Gold, D. P., Dreskin, S. C., Lernmark, Å, and Bellgrau, D. A Diabetogenic Gene Prevents T Cells from Receiving Costimulatory Signals. Cellular Immunology 194:90-97, 1999
5. Moore, J.K., Scheinman, R. I., and Bellgrau, D. The Identification of a Novel T Cell Activation State Controlled by a Diabetogenic Gene. J. Immunol. 166: 241-248, 2001
6. Salvador, J.M., Hollander, M.C., Nguyen, A.T., Kopp, J.B., Barisoni, L., Moore, J. K., Ashwell, J.D., Fornace, A.J. Jr. Mice Lacking the p53-Effector Gene Gadd45a Develop a Lupus-Like Syndrome. Immunity 4: 499-508, 2002
References Available Upon Request
