PPT Slide

THREE OR MORE COLOR

IMMUNOPHENOTYPING

1. IgG Block + MAB

wash

Biotin- second antibody F(ab')2

wash IgG Block + FL- MAB + PE-MAB + TC- Avidin

wash

2. IgG Block + FL-MAB + PE-MAB + B-MAB

wash

TC-Avidin

wash

3. IgG Block + FL-MAB + PE-MAB + TC-MAB wash

TC (third color) = PE/TR or PE/CY5 tandem or PerCP

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