MULTIPARAMETER IMMUNOFLUORESCENCE

Click here to start

Table of Contents

Reasons Antibodies do not bind to cells:

BLOCKING IS IMPORTANT

variation in gamma 1 myeloma protein binding to macrophages

DEAD CELLS CAN BE A PROBLEM

ANTIBODIES BIND NON-SPECIFICALLY TO DEAD CELLS

Evaluating Viability with Ethidium Monoazide

ONE COLOR IMMUNOPHENOTYPING

Analysis of List Mode Data

SINGLE COLOR HISTOGRAMS

TWO COLOR PATTERN

THREE COLOR PATTERN

FOUR COLOR SINGLE LASER IMMUNOPHENOTYPING

FOUR COLOR DUAL LASER IMMUNOPHENOTYPING

VISUALIZING EACH POPULATION

UNDERSTANDING BINARY LOGIC IS USEFUL

Non-specific Binding to Monocytes

EFFECT OF BATCH VARIATION

Author: Carleton C. Stewart, Sigrid J. Stewart, Roswell Park Cancer Institute

Email: stewart@sc3101.med.buffalo.edu

Back to Powerpoint slides index page