Three Color Lymphocyte Patterns
CD3
CD4
CD3
CD4
CD8
CD8
Data from Dr. Carleton Stewart
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Notes:
When more than two antibodies are combined, additional subsetting of the cells can be achieved. If one antibody is used to identify a specific lineage of cells, i.e. CD3, five subsets of T-cells are easily resolved, when CD4 vs C8 is displayed.
