Mechanics of a flow cell
How foward angle light scatter is collected
How 90 degree light scatter is collected
Fluorescence detection in the flow cell
Fluorescence activated cell sorting in the flow cytometer
Optical Filters
Mechanism of a band pass filter
Standard Long Pass Filters
Diagramatic representation of the optic layout of a flow cytometer
Common excitation and emission spectra of various fluorescent probes
Excitation and emission spectra of Propidium Iodide
Excitation and emission spectra of Fluorescein (FITC)
Excitation and emission spectra of Phycoerytherin (PE)
Excitation and emission spectra of Allophycocyanin (APC)
Typical Research Cytometer (Coulter 753) (1980s)
Diagram of typical clinical cytometer
The Conventional Microscope
Purdue University Cytometry Laboratories
Email: jpr@flowcyt.cyto.purdue.edu
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